cmoO [2018-02-09T23:33:26.000Z]

monooxygenase, required for the conversion of S-methyl-cysteine to cysteine

locus: BSU29330

pI: 5.84

mw: 36.79 kDa

protein length: 334 aa

gene length: 1002 bp

function: utilization of S-methyl-cysteine

product: monooxygenase

essential: no

synonyms: ytmO

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.1|Conversion of S-methyl cysteine to cysteine]

Gene

Coordinates: 3,003,345 → 3,004,349

Phenotypes of a mutant

• no growth with S-methyl cysteine [Pubmed|23944997]

The protein

Protein family

• Flavin-utilizing monoxygenases

Paralogous protein(s)

• [protein|1432F56AD3D2B2BD82D8759CE4DC42C0D40C49C0|YceB], [protein|A36E7D50E2EBD0F0109491FF2CAD2CA84372B2CF|YvbT], [protein|A1DCFF8D225C87197104742E72A0D4F238BC30EB|YwcH]

Structure

• [PDB|4US5] (from Streptomyces bottropensis, 31% identity) [pubmed|24554499]

Expression and Regulation

Operons

• genes: [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
  description: [Pubmed|15668000]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15272571], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|AscR]: activation, [Pubmed|16109943], in [regulon|741156D495BE3857683C8A0390764EAD83845ABC|AscR regulon]

  regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]

Biological materials

Mutant

• MGNA-A174 (ytmO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/174 NBRP B. subtilis, Japan]
• BKE29330 (Δ[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA
• BKK29330 (Δ[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA

References

• 16109943,15668000,15272571,23944997,24554499